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Plasma Metabolome Signature in Patients with Early-stage Parkinson Disease

[ Vol. 6 , Issue. 1 ]

Author(s):

Elena E. Balashova*, Petr G. Lokhov, Dmitry L. Maslov, Oxana P. Trifonova, Diana M. Khasanova, Zuleykha A. Zalyalova, Razina R. Nigmatullina, Alexander I. Archakov and Michael V. Ugrumov   Pages 75 - 82 ( 8 )

Abstract:


Background: Recent decades have been marked by advances in omics sciences based on high-throughput technologies, which have enabled the measurement of enormous numbers of molecules in biosamples. In metabolomics, a large number of small molecules (metabolome) can be detected in a single run. The goal of this study was to evaluate the capacity for metabolomic analysis of blood plasma for early-stage Parkinson Disease (PD) diagnosis.

Methods: Blood plasma samples collected from control subjects (n = 20) and patients with PD (Hoehn and Yahr stages 1, 1.5, and 2; n = 16) were treated with methanol, and low-molecular-weight fractions were analyzed by direct infusion mass spectrometry. Metabolite ions that exhibited strong association with PD were included in a diagnostic signature compilation and corresponding characteristics for PD diagnosis were calculated. For metabolite ions included in the signature, correspondence to specific metabolites in metabolite databases was established.

Results: A total of 21 metabolite ions that were strongly associated with PD were used to compile a metabolome signature. The area under a receiver operating characteristic curve (AUC) for PD diagnosis calculated for the signature was 0.95 (accuracy 94%, specificity 95%, and sensitivity 94%). Metabolites identified in this study were consistent with factors that had been associated with the development of PD previously.

Conclusion: Direct infusion mass spectrometry of blood plasma metabolites represents a rapid singlestep method with potential for application in early-stage PD diagnosis.

Keywords:

Metabolomics, parkinson disease, early diagnosis, patients, blood plasma, mass spectrometry.

Affiliation:

Institute of Biomedical Chemistry, Pogodinskaya st.10, 119121, Moscow, Institute of Biomedical Chemistry, Pogodinskaya st.10, 119121, Moscow, Institute of Biomedical Chemistry, Pogodinskaya st.10, 119121, Moscow, Institute of Biomedical Chemistry, Pogodinskaya st.10, 119121, Moscow, Republic Clinical Diagnostic Centre of Extrapyramidal Pathology and Botulinum Therapy, Ershova st. 65, 420061, Kazan, Kazan State Medical University, Butlerova st. 49, 420012, Kazan, Kazan State Medical University, Butlerova st. 49, 420012, Kazan, Institute of Biomedical Chemistry, Pogodinskaya st.10, 119121, Moscow, Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Vavilova st. 26, 119334, Moscow

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