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FTIR Spectroscopy Analysis can Highlight Induced Damage in Neuronallike Cells and Bio-protective Effectiveness of Agmatine

[ Vol. 6 , Issue. 1 ]

Author(s):

Nadia Ferlazzo, Emanuele Calabro, Monica Curro, Daniela Caccamo, Salvatore Magazu and Riccardo Ientile*   Pages 34 - 39 ( 6 )

Abstract:


Background: Agmatine, an endogenous amine, is cosidered a novel neuromodulator with neuroprotective properties. However, the mechanisms involved in these protective effects are poorly understood.

Methods: Fourier Transform Infrared (FTIR) spectroscopy analysis detects biomolecular changes in disordered cells and tissues. In the present study, we employ FTIR spectroscopy to characterize the changes in rotenone-induced damage in neuronal-like differentiated SH-SY5Y neuroblastoma cells in the presence or absence of agmatine.

Results: The analysis of the FTIR spectra evidences significant alterations in rotenone-treated cells that are reduced by the pre-incubation with agmatine (250 nM). In particular, rotenone-damaged cells demonstrate spectral alterations related to amide I, which correspond to an increase in β-sheet components, and decreases in the amide II absorption intensity, suggesting a loss of N-H bending and C-N stretching. These alterations were also evident by Fourier self-deconvolution analysis. Thus, rotenone induced increases in the levels of stretching vibration band related to the protein carboxyl group would account for a significant amount of misfolded proteins in the cell. Agmatine effectively reduces these effects of rotenone on protein structure.

Conclusion: In conclusion, antioxidant and scavenging properties of agmatine reduce rotenoneproduced cellular damage at the level of protein structure. Our results, together with other previous observations, make agmatine a potential therapeutic agent in the treatment of Parkinson's disease.

Keywords:

FTIR, mitochondrial impairment, neuron, oxidative stress, static magnetic fields, scavenging.

Affiliation:

Department of Biomedical and Dental Sciences and Morphological and Functional Images, University of Messina, Messina, Department of Mathematics and Computing, Physics and Earth Sciences, University of Messina, Messina, Department of Biomedical and Dental Sciences and Morphological and Functional Images, University of Messina, Messina, Department of Biomedical and Dental Sciences and Morphological and Functional Images, University of Messina, Messina, Department of Mathematics and Computing, Physics and Earth Sciences, University of Messina, Messina, Department of Biomedical and Dental Sciences and Morphological and Functional Images, University of Messina, Messina

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