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Background: Blood serum is characteristic mixture of naturally occurring endogenous fluorophores which are sensitive to endogenous and exogenous stress during physiological as well as pathological processes in the body.
Methods: Dynamic changes of blood serum in patients with positive troponin and creatine kinase during myocardial infarction compared to control blood serum of healthy subjects were studied by synchronous fluorescence fingerprint and atomic force microscopy at 0, 1st, 16th, and 24th hour time points.
Results: While creatine kinase and troponin T values were in physiological range, fluorescence intensity of patient''s blood serum was significantly increased at λ = 280 nm, p < 0.001 at the first zero time point. In addition, at spectrum record appeared a second fluorescence peak place at λ = 306 nm in comparison with control blood serum fluorescence. Blood serum proteins changed structure from globular to fibrils during acute myocardial infarction in early 0 and 1st hour timepoints in comparison to control samples with regularly arranged globular proteins studied by atomic force microscopy. Otherwise, creatine kinase and troponin T values were positive in blood serum of patients several hours after beginning of acute myocardial infarction while fluorescence intensity exhibited decrease. Conversion of fibrils to irregular bigger globular proteins in comparison to blood serum with regular smaller globular proteins in control during later 16th and 24th hour time points was observed.
Conclusion: Mentioned new nontraditional techniques may be used in future for the early study of progress of the various cardiovascular diseases in patients with differential diagnosis which in daily practice meets some difficulties.
Atomic force microscopy, blood serum, dynamic progress of ischemia, endogenous stress factors, hypoxia, myocardial infarction, proteins, synchronous fluorescence spectroscopy.